بررسی فنوتیپ ها و فعالیت مهاری الفا - یک آنتی تریپسین در بیماران آمفیزم

ABSTRACT Alpha 1-Antitrypsin is one of the most important serine protease in human serum,inhibiting several proteases.As an inhibitor of proteases it is a non-specific lever in humoral defence of the body. Its level increases significantly under the infections condition of pregnancy. The health of lung,too, depends on maintaininig the equilibrium of protease antitrypsin ,and Alpha 1-trypsin plays an important role in this equilibrium. Thus,determination of its level in degenerative diseases of lung tissue is of great importance.Because of genetic diversity,this enzyme has numerous isoenzymes and it constitutes different phenotypes,depending on the type of the disease. Allele quantities of these antiproteases vary. In this examination, of 30 male patients presenting to the pulmonary Ward of Imam Khomeini Hospital in Tehran (mean age 47.37+18.47), blood sample was taken.Previously,Pulmonary emphysema was confirmed in these patients by their physicians. Of 30 male volunteers with mean age of 30.73+5.39,blood sample was taken. TIC was measured by spectrometry and by an appropriate substrate. Phenotypes, also,were determined with assistance of the standard serum prepared by World Reference Laboratory of Alpha 1-Antitrypsin in Norway.The results indicate that the rate of TIC in patients and in control individuals has a significant difference(P<0.05).Determination of phenotypes suggest consistency of phenotypes as well as the alleles of patients and the controls. Therefore,it can be concluded that,firstly,since all patients had normal phenotype, the role of genetic factors in occurance of emphysema isn't dramatic.Secondly, despite the high rate of TIC in patients, phenotypes are normal .Thus through the rate of TIC ,one can not determine the phenotypes accurately and more reliable procedures such as focal electrophoresis are required.